My lab utilises state-of-art multi-omics methodologies to study how protein synthesis is dysregulated in cancer cells, and how this dysregulation contributes to various stages of cancer progression.
Methods for monitoring and measurement of protein translation in time and space. Mol Biosyst (2017) 13(12):2477-2488. PMID: 29051942
Global Analysis of mRNA, Translation, and Protein Localization: Local Translation Is a Key Regulator of Cell Protrusions. Dev Cell (2015) 35(3):344-5. PMID: 26555054
Rho-associated kinase (ROCK) function is essential for cell cycle progression, senescence and tumorigenesis. Elife (2016) 5:e12994. PMID: 26765561
Proteomics profiling of interactome dynamics by colocalisation analysis (COLA). Mol Biosyst (2016) 13(1):92-105. PMID: 27824369
Each cell in our body contains the same genetic information, yet this information is read out in diverse manners, allowing different cell types to acquire distinct characteristics.
Importantly, the decoding of genetic information can be corrupted in diseases such as cancer, resulting in undesired characteristics, such as unrestricted proliferation and invasion of malignant cells into other tissues and organs.
The decoding of genetic information occurs sequentially from DNA, via messenger-RNA (mRNA), to proteins, the final and functional products of most genes. My lab is particularly interested in understanding how synthesis of cellular proteins from mRNA (i.e. protein translation) is dysregulated in cancer cells.
Using cutting-edge quantitative proteomics, RNA-seq, and bioinformatics methodologies, along with a variety of cell based and biochemical assays, we study how protein translation is controlled in time and space, and how this regulation is altered during cancer progression.
Currently, the lab is focused on four projects:
If you are interested in working/studying in our lab, please e-mail me.
DNA repair deficiency sensitizes lung cancer cells to NAD+ biosynthesis blockade. Touat M, Sourisseau T, Dorvault N et al. J Clin Invest (2018) 128(2) 1671-1687
Mass Spectrometry Analysis of Spatial Protein Networks by Colocalization Analysis (COLA). Mardakheh FK (2017) 1636(2) 337-352
Methods for monitoring and measurement of protein translation in time and space Dermit M, Dodel M, Mardakheh FK Molecular BioSystems (2017) 13(7) 2477-2488
RHO binding to FAM65A regulates Golgi reorientation during cell migration. Mardakheh FK, Self A, Marshall CJ J Cell Sci 129(1) 4466-4479
NAMPT inhibition is a novel synthetic lethal therapeutic approach exploiting nuclear-mitochondrial crosstalk in ERCC1-deficient populations Touat M, Olaussen K, Sourisseau T et al. EUROPEAN JOURNAL OF CANCER (2016) 69(11) S56-S56
Proteomics profiling of interactome dynamics by colocalisation analysis (COLA). Mardakheh FK, Sailem HZ, Kümper S et al. Mol Biosyst (2016) 13(1) 92-105
Microenvironmental Heterogeneity Parallels Breast Cancer Progression: A Histology-Genomic Integration Analysis. Natrajan R, Sailem H, Mardakheh FK et al. PLoS Med (2016) 13(2) e1001961
Rho-associated kinase (ROCK) function is essential for cell cycle progression, senescence and tumorigenesis. Kümper S, Mardakheh FK, McCarthy A et al. Elife (2016) 5(2) e12994
Global Analysis of mRNA, Translation, and Protein Localization: Local Translation Is a Key Regulator of Cell Protrusions. Mardakheh FK, Paul A, Kümper S et al. Dev Cell (2015) 35(2) 344-357
Rho kinase inhibitors block melanoma cell migration and inhibit metastasis. Sadok A, McCarthy A, Caldwell J et al. Cancer Res (2015) 75(2) 2272-2284
Dr Maria Dermit
Mr Martin Dodel
Mr Muhammad Syahmi Bin Azman, Mr Elliott Whittaker