My lab utilises state-of-art multi-omics methodologies to study how protein synthesis is dysregulated in cancer cells, and how this dysregulation contributes to various stages of cancer progression.
An ERK1/2-driven RNA-binding switch in nucleolin drives ribosome biogenesis and pancreatic tumorigenesis downstream of RAS oncogene. EMBO Journal (2023) e110902. PMID: 37039106
Purification and quantitative proteomic analysis of cell bodies and protrusions. Star Protocols (2021) 2, 2, 100462. PMID: 33912849
A prometastatic splicing program regulated by SNRPA1 interactions with structured RNA elements. Science (2021) 14;372(6543). PMID: 33986153
Subcellular mRNA localization regulates ribosome biogenesis in migrating cells. Dev Cell (2020) 55(3):298-313.e10. PMID: 33171110
Methods for monitoring and measurement of protein translation in time and space. Mol Biosyst (2017) 13(12):2477-2488. PMID: 29051942
Each cell in our body contains the same genetic information, yet this information is read out in diverse manners, allowing different cell types to acquire distinct characteristics. Importantly, the decoding of genetic information is often corrupted in cancer, resulting in undesired characteristics, such as unrestricted proliferation and invasion of malignant cells into other tissues and organs.
The decoding of genetic information occurs sequentially from DNA, via messenger-RNA (mRNA), to proteins, the final and functional products of most genes. My lab is particularly interested in understanding how synthesis of cellular proteins from mRNA is dysregulated in cancer cells through the action of an important group of proteins known as RNA Binding Proteins (RBPs).
Using cutting-edge quantitative proteomics, RNA-seq, and bioinformatics methodologies, along with a variety of cell based and biochemical assays, we study how RBPs regulate RNA in time and space, and how this regulation is altered during cancer progression.
Currently, the lab is focused on four projects:
Ultimately, our goal is to understand how various aspects of cancer progression are mediated by dysregulation of RBPs, in hope of revealing novel therapeutic targets that can be exploited to tackle malignancy.
If you are interested in working/studying in our lab, please e-mail me.
An ERK1/2-driven RNA-binding switch in nucleolin drives ribosome biogenesis and pancreatic tumorigenesis downstream of RAS oncogene Azman MS, Alard EL, Dodel M et al. EMBO Journal (2023) (7)
An mRNA processing pathway suppresses metastasis by governing translational control from the nucleus Navickas A, Asgharian H, Winkler J et al. Nature Cell Biology (2023) (7)
ERG activity is regulated by endothelial FAK coupling with TRIM25/USP9x in vascular patterning D'Amico G, Fernandez I, Gómez-Escudero J et al. Development (Cambridge) (2022) 149(7)
Targeted therapy for LIMD1-deficient non-small cell lung cancer subtypes Davidson K, Grevitt P, Contreras-Gerenas MF et al. Cell Death and Disease (2021) 12(7)
Adipocytes disrupt the translational programme of acute lymphoblastic leukaemia to favour tumour survival and persistence Heydt Q, Xintaropoulou C, Clear A et al. Nature Communications (2021) (1)
A preclinical pipeline to evaluate migrastatics as therapeutic agents in metastatic melanoma Maiques O, Fanshawe B, Crosas-Molist E et al. British Journal of Cancer (2021) 125(7) 699-713
The elongation factor eEF1A2 controls translation and actin dynamics in dendritic spines Mendoza MB, Gutierrez S, Ortiz R et al. Science Signaling (2021) 14(7)
Purification and quantitative proteomic analysis of cell bodies and protrusions Dermit M, Mardakheh FK STAR Protocols (2021) 2(7)
A prometastatic splicing program regulated by SNRPA1 interactions with structured RNA elements Fish L, Khoroshkin M, Navickas A et al. Science (2021) 372(7)
Centrosome amplification mediates small extracellular vesicle secretion via lysosome disruption Adams SD, Csere J, D'angelo G et al. Current Biology (2021) 31(7) 1403-1416.e7For additional publications, please click here
Dr Alina Chakraborty
Mr Martin Dodel
Mr Muhammad Syahmi Bin Azman, Mr Elliott Whittaker, Ms Federica Capraro, Ms Emilie Alard