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Pancreatic cancer cells (PANC-1) were grown into three-dimensional spheroids in a biomimetic hydrogel matrix for 14 days, then stained with DAPI (nuclei, blue), phalloidin (cytoskeleton, magenta), KRT19 (cancer marker, green). Spheroids were imaged by confocal microscopy and pictures were merged to create the image on the right. Top and bottom spheroids were created with individual colour overlay in ImageJ. The team aim to use these 3D models to better the understanding of cancer mechanobiology. Image by Eleonora Peerani, former BCI PhD student.