Contact

Julfa Begum
Flow Cytometry Facility Manager

+44 20 7882 2119

Flow Cytometry

This service is equipped with flow cytometry, mass cytometry and imaging cytometry instrumentation enabling rapid, multiparametric analysis across a number of disciplines.

Mass Cytometry

Phenotypic analysis of single cells using Time of Flight Mass Spectrometry

ImageStream X MkII

Imaging Cytometry combines high throughput flow cytometry with imaging.

Cell Sorters

Multi-parameter fluorescent activated cell sorters to isolate and recover your cells of interest

Analysers

Multi-parameter analysis of fluorescently labelled cells

Internal users: please visit the Intranet

QMUL users: please contact Julfa Begum

For external users:

Equipment Rate per hour
Academic users Non-academic users
FACS Aria £75 £125
FACS Fusion £75 £125
FACS Fortessa £40 £60
ImageStream X Mk II £40 £60
CyTOF 2 £75 £150

Mass Cytometry

Phenotypic analysis of single cells using Time of Flight Mass Spectrometry

  • CyTOF 2: Provides next generation single cell analysis of cells in suspension, with the ability to investigate over 30 cellular parameters simultaneously. Mass Cytometry labels cells with antibodies conjugated to stable heavy metal isotopes in the place of fluorochromes reducing spill over and the need for compensation. This makes it the perfect tool for gaining a large amount of information where sampling material is limited, and for carrying out complex phenotyping of rare cell types.
  • Hyperion: Imaging Mass Cytometry harnesses the multiparametric power of mass cytometry in a slide-based format; permitting simultaneous interrogation of up to 37 markers of interest in both FFPE and frozen tissue sections.

ImageStream X MkII

Imaging Cytometry combines high throughput flow cytometry with imaging.

The ImageStream provides bright field and fluorescence microscopy images of each cell at 20x, 40x or 60x magnification. It is equipped with two CCD cameras and three lasers- blue (488nm), violet (405nm) and red (642nm)- which can be used to look at up to 10 different fluorescent parameters. It is ideal for investigating co-localisation, internalisation, immunological synapse and much more.

Cell Sorters

Multi-parameter fluorescent activated cell sorters to isolate and recover your cells of interest

  • Aria II: Consists of four lasers- violet (405nm), blue (488nm), yellow/green (561nm) and red (633nm)- and is capable of measuring up to 14 parameters. It is equipped to sort up to four cell populations simultaneously. It has the ability to sort into tubes, plates or slides.
  • Aria Fusion in a Biosafety Cabinet: Consists of three lasers- violet (405nm), blue (488nm) and red (633nm)- and is capable of measuring up to 14 parameters. It is equipped to sort up to four cell populations simultaneously. It has the ability to sort into tubes, plates or slides. The
    sorter is in a biosafety cabinet allowing the processing of unscreened human material.

Analysers

Multi-parameter analysis of fluorescently labelled cells
The facility consists of three Fortessas.

  • Two Fortessas are equipped with four lasers- violet (405nm), blue (488nm), yellow/green (561nm) and red (633nm)- and are capable of measuring up to 16 parameters.
  • One Fortessa is equipped with three lasers- violet (405nm), blue (488nm) and red (633nm)- and is capable of measuring up to 14 parameters.

Data analysis

  • FlowJo
  • Cytobank
  • Ideas
  • BD FacsDIVA
  • MCD Viewer