CRUK Barts Centre Pathology Service

Contact

Nadia Rahman
Histopathology Manager

+44 20 7882 3555

Pathology

The Pathology laboratory offers specialist histological services to Researchers at the CRUK Barts Centre. It is well equipped in the latest automated histological equipment which guarantees reliable and reproducible results and reduces general hazards and risks, ensuring that users are protected.
The service is also available to external users.


Requests for services for internal users are made using the online system - iLab Solutions.
You will find further information on how to use iLab with the following links:
- Internal users can register for Pathology services at https://qmul.corefacilities.org/account/596/signup or at https://eu.ilabsolutions.com/account/signup
- External users need to register for an external account and once this is done, they will need to submit a request which will be reviewed by the service. Further information can be found at https://help.ilab.agilent.com/99540-getting-started-with-ilab/325013-registering-with-ilab and https://help.ilab.agilent.com/37179-using-a-core/264646-using-a-core-overview.


Location: Ground floor, Room G03, John Vane Science Centre, Charterhouse Square, London EC1M 6BQ


New safety measures in place when entering the Pathology laboratory from 1st July 2020

The Pathology service is open to collaborations to develop new procedures/techniques for any potential new projects. This service is available to other Queen Mary departments and to external users.

Models
  • Tissue-Tek® VIP® 6 AI Vacuum Infiltration Processor
  • Leica TS5015 auto Stainer XL and coverslipper CV5030
  • Leica RM2255 rotary microtome
  • Leica RM2135 microtome
  • Leica Histocore AUTOCUT microtome
  • Leica CM3050 S cryostat
  • Leica CM1860: cryostat
  • Bright OTF 5000: cryostat
  • Leica EG1160: embedding Centre
  • Ventana Discovery XT: IHC auto Stainer
  • Ventana Discovery Ultra: IHC auto Stainer
Services

The following must be for medical research purposes only:

1. Fixed tissue: tissue processing by automation and embedding into paraffin blocks.

2. Section cutting on Palm/membrane slides, Mega slides or adhesive/positively charged microscope glass slides (75x25mm and 1mm thick) at various thickness (usually from 3 to 10 microns, standard being at 4 microns):

  • Serial sections

  • Single or multiple sections per slides

  • Large sections (on mega slides)

  • Cutting under DNase/RNase free conditions for DNA/RNA extraction and collection on standard microscope glass slides

  • Cutting of ribbons of paraffin sections collected in sterile Eppendorf tubes under DNase/RNase free conditions for DNA/RNA extraction

3. Cryoblock from Frozen tissue:

  • Serial sections

  • Single or multiple sections per slides

  • Cutting under DNase/RNase free conditions for DNA/RNA extraction and collection on positively charged (extra adhesive) microscope glass slides

  • Cutting of ribbons of paraffin sections collected in sterile Eppendorf tube under DNase/RNase free conditions for DNA/RNA extraction

4. Staining:

  • Hematoxylin & Eosin (H&E) automated staining of frozen and paraffin sections (sections on standard and mega glass slides)

  • Special stains: Sirius Red, Neutral Red, AB/PAS, PAS, Oil Red O

5. Automated Immunohistochemistry (IHC) staining using on Ventana Discovery XT and Ultra machines using: 

  • DAB Map kit (basic kit) and Omni Map MsHRP & RbHRP

  • This includes optimisation of new biomarkers (using anti-human, anti-mouse primary antibodies, various secondary antibodies for testing on xenografts, mouse or human tissue sections, strictly for medical research purposes (non-diagnostic purposes).

  • Please note, the facility does not have archival animal and human tissue available as control material for optimisation so it is advisable that users make a request to the appropriate tissue bank if necessary and provide their control material when submitting their request.

 

Internal users: please visit the Intranet

QMUL users: please contact Nadia Rahman

For external users: please contact Nadia Rahman

The Pathology Core Service is equipped with a wide range of carefully selected instruments, which undergo regular maintenance visits exclusively made by engineers who are manufacturers' trained and approved in order to ensure reliability, consistency and reproducibility of results at all times.

Automation of all the steps is achieved, from tissue processing, sectioning of paraffin and cryo blocks to Hematoxylin and Eosin (H&E) and Immunohistochemistry staining. View the document below for full model details.

CRUK Barts Centre Pathology Service - Model Details